subject details primary cell cultures primary fibroblasts (ATCC)
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Subject Details Primary Cell Cultures Primary Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 582 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 582 article reviews
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1) Product Images from "MicroRNAs Induce a Permissive Chromatin Environment That Enables Neuronal Subtype-specific Reprogramming of Adult Human Fibroblasts"
Article Title: MicroRNAs Induce a Permissive Chromatin Environment That Enables Neuronal Subtype-specific Reprogramming of Adult Human Fibroblasts
Journal: Cell stem cell
doi: 10.1016/j.stem.2017.08.002
Figure Legend Snippet: (A) Genome wide expression analysis of miNs and starting fibroblasts by RNA-seq at day 30 of reprogramming. Plot shows the relationship between average gene expression (logCPM) and log fold-change of miNs compared to fibroblasts. A selection of pan-neuronal and fibroblast-specific genes are highlighted in black text. Blue = fold change < −2 log2 p < 0.01 (more abundant in fibroblasts), grey = fold change > −2 log2 < 2 log2 p > 0.01, and red = fold change > 2 log2 p < 0.01 (more abundant in miNs). FDR < 0.01.
Techniques Used: Genome Wide, Expressing, RNA Sequencing, Gene Expression, Selection
Figure Legend Snippet: (A) Schematic of sample collection during miR-9/9*-124-mediated neuronal reprogramming for DNA methylation studies. Human fibroblasts were transduced with virus expressing miR-9/9*-124 or a non-specific (NS) control (Ctrl) virus at day 0. Samples were collected at day 10, day 20, and day 30.
Techniques Used: DNA Methylation Assay, Transduction, Virus, Expressing, Control